Optimization of Culture Conditions for a New Mutant Penicillium EZ-ZH390 to Increase Tannase Activity in Submerged Fermentation
Increase tannase activity in submerged culture by Penicillium
Keywords:
Fermenter, Penicillium EZ -ZH390, submerged fermentation , tannase , walnut hullsAbstract
Back Ground and Aims: Tannins have some undesirable impact and should be broken down by tannase enzymes. One of the major problems of microbial produced tannase is the high total cost which could be reduced by using agricultural waste as a substrate.
Method: A submerged fermentation system was used to optimize production of tannase from newly isolated Penicillium EZ-ZH390. Dried walnut hulls were used as carbon substrate. Statistical design screening was used to find main variables. In the next step, these three factors were used to optimize tannase using response surface methodology.
Results and Discussion: According to the statistical design screening results, shaking, spore inoculum amount, and temperature had the greatest impact on tannase activity. Without optimization, the activity of tannase in walnut hull medium was 0.36 U mL-1. A shaking speed of 180 rpm and an inoculum of 108 cfu mL-1 spores at 25°C were obtained as the optimal conditions using the model. It was also examined whether aeration affects tannase activity and growth kinetics of Penicillium EZ-ZH390 in the fermenter as well as the enzyme activity over time. The aeration 1vvm produced the greatest effect on tannase activity. The highest enzyme activity was observed between hours 60 and 75. As total sugars, reducing sugars, and tannin levels continue to decrease, Penicillium EZ-ZH390 is growing. Tannase production from Penicillium EZ-ZH390 in walnut hulls submerged fermentation medium is dependent on microorganism growth. The highest enzyme activity measured using this method was 3.05 U mL-1.
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