Development of Antibody-Imprinted PolymericNanomembranes for Rapid and Selective Detection ofStaphylococcus aureus
Nanomembranes for Rapid and Selective Detection of S. aureus
Keywords:
Staphylococcus aureus detection, , Molecularly Imprinted Polymer, Nanomembranes, Antibodyimprinted polymers, Selective bacterial capture, Food safety, Clinical diagnosticAbstract
Identification and isolation of Staphylococcus aureus is of great importance in clinical and food applications, but traditional methods face several drawbacks. Our current investigation focused on developing and evaluating Molecularly Imprinted Polymer (MIP)-functionalized nanomembranes for selective S. aureus capture. MIPs were synthesized on cellulose acetate membranes via UVinitiated polymerization. Characterization via FTIR confirmed antibody integration, while SEM revealed distinct MIP nanoparticles (20-45 nm) compared to larger non-imprinted polymer (NIP) particles (295-2132 nm). Filtration experiments using S. aureus suspensions (10⁴-10⁵ CFU/mL) demonstrated the membranes' capture capability; notably, filter M3 reduced a 3 × 10⁵ CFU/mL challenge concentration to 4.3 × 10⁴ CFU/mL in the filtrate. Performance varied across formulations, with differences in filtration times and retention efficiencies observed between MIP and NIP filters. To enhance consistency, further optimization of monomer-to-template ratios is recommended. The MIP filters exhibited robust stability over a two-month evaluation period. These findings highlight the potential of antibody-imprinted MIP nanomembranes as promising tools for S. aureus capture, offering a rapid and selective alternative approach that warrants further optimization and testing in complex matrices for practical applications.
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Copyright (c) 2025 Sogand Heydari (Author); Hamed Hamed Ahari; Seyed Amirali Anvar, Artemiss Mojalal, Sima Moradi (Author)
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.