The production of Poly (3-Hydroxybutyrate) in the extremely halophilic archaea Halarchaeum acidiphilum
Keywords:
biodegradable plastics, crotonic acid, halophiles, polyhydroxybutyrateAbstract
Biodegradable Polyhydroxyalkanoates (PHAs), produced by bacteria, are a solid alternative to native plastic. However, in the archaea domain, some members of the haloarchaea genera have been identified as PHAs producers. In the present study, we selected Halarchaeum acidiphilum strain and optimized it for PHB production.
We isolated the Halarchaeum acidiphilum strain from the Lut desert of Iran and performed Sudan black stain and UV absorption of crotonic acid. The results showed that the strain produced PHA in the medium containing 12.5% sodium chloride and pH equal to 7.6 at 37°C at the stationary phase. The PHA was extracted in sterile water, and the Physico-chemical analysis of PHA was detected by FTIR and 1HNMR test, which approved the ester structure and the efficiency was almost 71%.
The use of haloarchaea in the production of PHA has advantages such as no need for sterile conditions, simple raw materials, and fast lysis of bacteria in the absence of salt, which help the processes of the economic cycle of PHAs extraction.
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Copyright (c) 2026 Sheivan Chahianchy (Author); Soheila Abbasi; Giti Emtiazi, Maryam Jalili Tabaii, Martin Koller (Author)
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