PCR Identification of Subtilosin and Coagulin synthetase gene in Bacillus coagulans, Cultured in an Artificial Medium
Identification of subtilosin and coagulin synthetase gene in B. coagulans
Keywords:
Bacillus coagulans, Coagulin, Subtilosin, PCR identificationAbstract
Purpose and aim: Bacillus spp. is particularly important among probiotic microorganisms due to its resistance to extreme conditions. Bacillus coagulans, producing coagulin is a probiotic bacteria used in food products. This study aimed to genetically identify bacteriocins in B. coagulans IBRC-M 10807.
Method: The agar-well diffusion method was used to examine the antimicrobial property of the 24-hour crude supernatant. The heat and storage resistance of the crude supernatant was studied, and the HPLC technique was utilized to analyze its percentage of amino acids. Finally, the PCR method detected the presence of coagulin, subtilin, and subtilosin genes.
Results and conclusion: The results showed the antimicrobial properties of the CS against gram-positive/gram-negative bacteria. The best storage condition for the CS was -20 °C for 4 weeks, and it was resistant to <50 °C for 30 minutes. Furthermore, the artificial medium in this study with similar efficiency and a lower price, was valuable compared to the other two commercial media. PCR results showed the presence of a 3000-3500 bp band related to coagulin (observed only after plasmid extraction) and a 250-300 bp band related to subtilosin after sequencing. The sequence of the subtilosin band had 100% identity with the B. subtilis strain L4 subtilosin (albA) gene. These broad-spectrum antimicrobial properties, and their relative resistance to temperature and storage, make this strain a valuable candidate for use in probiotic products.
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Copyright (c) 2026 Elham Mousavi , Maryam Jalili Tabaii , Fatemeh Sadat Ghoreishi , Morteza Abkar , Giti Emtiazi (Author)
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